Research Associate II The Vector Engineering Lab

The Vector Engineering laboratory led by Ben Deverman is looking for a research associate to join our team. We are a fast-paced and mission-driven collaborative laboratory focused on developing next-generation adeno-associated viral (AAV) vectors for neuroscience research and for CNS gene this role you will assist with the execution of in vitro and in vivo studies aimed at developing a brain-wide gene therapy for a genetically defined neurodegenerative disease. For examples of our recent work please see the references at the end of the job description.

Success in this role will require that you are meticulous with your lab work and documentation and that you are able to work closely with a team of wet-lab and computational scientists to assure the timely completion of the teams research objectives. You will be expected to function independently in your daily responsibilities after training. Opportunities for presenting your work to your colleagues will be provided.

CHARACTERISTIC DUTIES

Culturing human embryonic stem cell-derived neurons

Molecular biology techniques such as DNA and RNA purification PCR qPCR and analysis of proteins

IP and IV injections in rodents

Tissue sectioning and cryo-sectioning

Immunohistochemistry staining of sectioned tissues and fluorescence microscopy

Extraction and purification of RNA and DNA from mammalian tissue samples

Organization tracking and storing of samples in house and with external repositories

Consulting colleagues and the scientific literature as needed to develop professional expertise and support ongoing and future projects

Troubleshooting to resolve problems and participate in new technology evaluation and implementation

Maintenance of meticulous records and documentation compilation and analysis of experimental data

Ordering and maintenance of stocks of reagents and supplies

Attendance and presentation at team meetings to share results plan projects and experiments and ensure that projects support team goals

QUALIFICATIONS

B.S. or M.S. in a biology related field

Experience handling mice or other small laboratory animals

Hands-on experience with extracting DNA and/or RNA from cells or tissues

Foundational knowledge of (and some hands on experience with) PCR and other common molecular biology techniques

Hands-on experience with mammalian cell culture

Experience working in a BL2 or BL2 environment

Exceptional organizational and time management skills electronic lab notebook practices and careful attention to detail

Outstanding interpersonal and communication skills and attention to detail

Strong work ethic reliability and desire to work in a team

Publications to review:

expected base pay range for this position as listed above is based on a 40 hour per week schedule. Broad provides pay ranges representing its reasonable and good faith estimate of what the organization reasonably expects to pay for a position at the time of posting. Actual compensation will vary based on factors including but not limited to relevant skills experience education qualifications and other factors permissible by law.