Laboratory Technician

Work Location

IC: NIAID Street: 9000 Rockville Pike Bldg: NIHBC 10 - CRC Room: B3-4233 City: Bethesda State & Zip: MD 20892

Position Requirements

Overall Position Summary and Objectives

The Contractor shall support studies on the molecular and cellular basis of cerebral toxoplasmosis in a mouse model of infection. The project is focused on the chemokine receptor Ccr7 in its protective role against cerebral toxoplasmosis. It involves oral infection of susceptible mice with Toxoplasma gondii cysts with subsequent monitoring of mortality weight loss organ pathology immune cell infiltrates and cytokine production.

Min Education - Bachelors

Resume Max Pages 3


Additional Qualifications

Certifications & Licenses - N/A

Field of Study - Animal Sciences

Software - Outlook

Skills

- Support studies on the molecular and cellular basis of cerebral toxoplasmosis in a mouse model of infection

Statement of Work Details

Plans executes and arranges laboratory experiments new techniques and procedures; and performs laboratory techniques relating to scientific research.

• Perform fundamental procedures required for research projects.
• Perform routine experiments and tests including tissue culture cell separation protein and quantitative and qualitative analysis.
• Follow established procedures and protocols; utilize aseptic technique to perform experiments.
• Perform a variety of laboratory techniques and procedures.

Operates laboratory machines and instruments; and prepares specimens and equipment for use.

• Work with staff on processing specimens for embedding according to protocols.
• Prepare materials and equipment for experiments.
• Work with a variety of routine and specialized laboratory instruments and equipment in the laboratory.

Performs sample management and storage.

• Store new samples update database retrieve samples with proper authorization.
• Upkeep sample and database inventory; monitor overall sample inventory.
• Retrieve catalogue and process samples including but not limited to blood and tumour tissue.

Analyzes documents and reports on all procedures performed.

• Convey and discuss research results with team lab members.
• Update and maintain written laboratory record of the work.
• Collect and enter research data into laboratory notebooks and databases.

Maintains inventory of laboratory supplies.

• Ensure that supplies and consumables are ready and on hand as necessary for maximum efficiency in the laboratory.

Cleans and sterilizes instruments glassware and equipment.

• Work with staff to cover glassware washing media preparation and autoclaving.

Plan execute and arrange laboratory experiments new techniques and procedures; and performs laboratory techniques relating to scientific research.

• Perform fundamental procedures required for research projects.

Maintains inventory of laboratory supplies and initiates purchase orders.

• Monitor and inventory routine laboratory supplies; ensure that supplies are stocked and organized.

Performs routine equipment maintenance.

• Perform laboratory maintenance tasks including scheduling liquid waste container pickup.

Plan execute and arrange laboratory experiments new techniques and procedures; and performs laboratory techniques relating to scientific research.

• Perform fundamental procedures required for research projects. 5

Analyzes documents and reports on all procedures performed and produces detailed reports for use by senior staff.

• Provide experimental progress updates on procedures performed in the laboratory.

Performs advanced equipment maintenance.

• Perform laboratory maintenance tasks including scheduling liquid waste container pickup. 3

Trains staff in performing routine laboratory tests and procedures.

• N/A

Provides insights and expertise and recommends new strategies and approaches.

• Engage in problem solving as required to solve technical difficulties and to ensure steady progress with a goal of providing high-quality experimental data suitable for publication. 1
• Evaluate new types of experimental approaches/analyses and recommend changes to protocols. 2

Deliverables

• Meet with lab members to present updates Weekly