ScientistSr Scientist

San Carlos, CA - USA

Monthly Salary: Not Disclosed

Posted on: 7 hours ago

Vacancies: 1 Vacancy

Job Summary

Company Overview
TwoStep Therapeutics is a biotechnology company developing a pipeline built around a unique tumor-targeting polyspecific integrin-binding peptide (PIP) that can selectively bind several targets that are highly expressed on solid tumors. This multi-targeting feature enables the agents broad applicability to a wide range of tumor types and patient populations overcoming the limitations of single-antigen targeting approaches. The company has leveraged this technology to create a pipeline of targeted cancer therapies for cytotoxic drug delivery and radioligand therapy. TwoStep Therapeutics was co-founded by CEO Caitlyn Miller in collaboration with leading academic entrepreneurs at Stanford University including Nobel Laureate Carolyn Bertozzi Jennifer Cochran and Ronald Levy.

For more information visit or download our 2025 scientific poster (linked here).

Position Overview
We are seeking a driven and versatile Scientist/Sr Scientist to join our preclinical R&D team to help advance the next generation of targeted cancer therapeutics. This is a unique opportunity to contribute across multiple stages of drug developmentfrom early-stage discovery and lead optimization to the development of robust translatable assays that support clinical advancement of our growing therapeutic portfolio.

The ideal candidate is a highly independent hands-on scientist who thrives in a fast-paced biotech environment has a diverse technical skillset suited to multiple aspects of early-stage drug development and is willing to contribute across interdisciplinary roles.

Key Responsibilities

Translational Biomarker Assay Development

Design and develop immunohistochemistry (IHC) assays for target expression and biomarker analysis with an emphasis on clinical translatability
Establish robust protocols for IHC assay optimization including antibody selection antigen retrieval and signal amplification

Early Discovery and Lead Optimization

Develop optimize and execute in vitro assays to evaluate target engagement potency and selectivity of early pipeline candidates
Apply flow cytometry protein-binding assays and cell-based potency assays to guide decision making on pipeline progression
Participate in project teams to integrate in vitro findings with in vivo and translational data packages

General Scientific Contributions

Independently plan and execute experiments analyze data and present results to key stakeholders in a clear and timely manner
Maintain thorough accurate experimental records
Contribute to the preparation of scientific reports regulatory documents and presentations
Ensure compliance with all safety and regulatory requirements

Essential Qualifications

PhD in a relevant field (Biology Bioengineering Cell Biology Biochemistry Pharmacology Pathology etc.) or equivalent industry experience
Demonstrated hands-on expertise in IHC assay development and optimization
Proficiency in mammalian cell culture and cell-based functional assays
Experience with multi-color flow cytometry and microscopy
Strong analytical and problem-solving skills with the ability to interpret complex datasets and communicate findings clearly
Proven ability to manage multiple projects simultaneously in a collaborative environment
Excellent written and verbal communication skills with experience presenting data to cross-functional teams

Preferred Qualifications

Experience in oncology drug discovery or development particularly in solid tumors
Familiarity with translational biomarker strategy and clinical assay validation
Familiarity with integrin biology drug conjugates and peptide-based therapeutics
Prior experience in a small biotech or startup environment with demonstrated adaptability

What We Offer
At TwoStep Therapeutics we envision a future where every cancer patient has access to targeted safe and effective therapeutic options. We prioritize a diverse inclusive work environment that fosters personal and professional growth. Our comprehensive benefits include:

Competitive base salary and equity compensation
Unlimited Paid Time Off (PTO)
Comprehensive medical dental and vision benefits for individuals and families
401(k) plan
Company-paid basic life insurance

Job title and compensation are dependent on the candidates experience and skillset.

Equal Opportunity Employer
TwoStep Therapeutics is an equal opportunity employer. We prohibit unlawful discrimination based on race color religion gender sexual orientation gender identity/expression national origin/ancestry age mental/physical disability medical condition marital status veteran status or any other characteristic protected by law. If you require assistance in submitting an application due to incompatible assistive technology or a disability please contact us at We are committed to accommodating your needs promptly.

Join Us
If you are passionate about advancing cancer therapies and want to make a meaningful impact in the field we invite you to apply for this position at TwoStep Therapeutics. Together we can shape the future of cancer treatment.

Required Experience:

Senior IC

Company OverviewTwoStep Therapeutics is a biotechnology company developing a pipeline built around a unique tumor-targeting polyspecific integrin-binding peptide (PIP) that can selectively bind several targets that are highly expressed on solid tumors. This multi-targeting feature enables the agents...