Scientist II, Complex In Vitro Models

Worcester, MA - USA

Monthly Salary: Not Disclosed

Posted on: 19 hours ago

Vacancies: 1 Vacancy

Job Summary

AbbVie is seeking an experienced scientist to join our Immunology team to develop characterize and qualify complex in vitro models (CIVMs). This lab-based individual will be responsible for duties that include but are not limited to: routine cell culture for cell lines and primary cells creation and maintenance of complex in vitro models (e.g. organoid organ-on-chip) for multiple different tissue types with a focus on modeling autoimmune diseases and performing quantitative endpoint assays. This is an ideal position for a candidate who wants to be at the forefront of 3D tissue modeling technologies and can thrive in a diverse research environment; strong organizational multi-tasking collaboration and communication skills are critical.

Key Responsibilities:

Routine culture and maintenance of immortalized stem and primary cell cultures both in standard formats as well as organoids or organ-on-chip formats. Experience with these systems and with a variety of cell types (e.g. endothelial cells intestinal organoids fibroblasts immune cells etc.) is highly desirable.
Isolation of primary human cells including the isolation of primary cells from tissue and whole blood.
Designing and conducting experiments to characterize in vitro models including assessments of in vitro potency cytotoxicity transcript/protein abundance using techniques such as viability assays microscopy histopathology and transcriptomics/proteomics.
Conducting experiments with a high level of independence and initiative and performing tasks with minimal direct supervision for routine methods.
Independently schedule and prioritize workload to manage several tasks simultaneously and identify / utilize resources outside of your own group.
Designing experiments to characterize models generating foundational datasets to guide model selection for specific drug program needs.
Maintaining detailed electronic documentation to ensure adherence to departmental procedures and contribute to final reports as needed.

Qualifications :

BS or MS degree (or equivalent) in cell or molecular biology bioengineering or a related field with typically 7 years (BS) or 5 years (MS) of experience.
Experience with standard cell and molecular biological assays such as ELISA/Luminex nucleic acid extraction qPCR immunofluorescence staining and confocal microscopy cell proliferation/viability assays cytotoxicity assays flow cytometry / FACS.
Experience with primary-derived cell culture differentiation methodologies; phenotypic and functional characterization of tissue-specific and/or immunological cells; understanding of key biomarkers for core tissue cell populations and/or tissue-resident or blood-derived immune cell populations.
Excellent organizational and analytical skills with the ability to independently troubleshoot optimize and innovate experimental approaches.
Excellent communication skills with an ability to work collaboratively in a matrixed research environment.

Preferred Qualifications:

Experience working with human tissue samples and with isolating and characterizing cells such as epithelial endothelial and/or immunological (B cells T cells monocytes).
Familiarity with automation or high-throughput screening if relevant as these are becoming increasingly important in advanced in vitro modeling.

Additional Information :

Applicable only to applicants applying to a position in any location with pay disclosure requirements under state or local law:

The compensation range described below is the range of possible base pay compensation that the Company believes in good faith it will pay for this role at the time of this posting based on the job grade for this position. Individual compensation paid within this range will depend on many factors including geographic location and we may ultimately pay more or less than the posted range. This range may be modified in the future.
We offer a comprehensive package of benefits including paid time off (vacation holidays sick) medical/dental/vision insurance and 401(k) to eligible employees.
This job is eligible to participate in our short-term incentive programs.

Note: No amount of pay is considered to be wages or compensation until such amount is earned vested and determinable. The amount and availability of any bonus commission incentive benefits or any other form of compensation and benefits that are allocable to a particular employee remains in the Companys sole and absolute discretion unless and until paid and may be modified at the Companys sole and absolute discretion consistent with applicable law.

AbbVie is an equal opportunity employer and is committed to operating with integrity driving innovation transforming lives and serving our community. Equal Opportunity Employer/Veterans/Disabled.

US & Puerto Rico only - to learn more visit & Puerto Rico applicants seeking a reasonable accommodation click here to learn more:

Work :

Employment Type :

Full-time

Key Responsibilities:

Routine culture and maintenance of immortalized stem and primary cell cultures both in standard formats as well as organoids or organ-on-chip formats. Experience with these systems and with a variety of cell types (e.g. endothelial cells intestinal organoids fibroblasts immune cells etc.) is highly desirable.
Isolation of primary human cells including the isolation of primary cells from tissue and whole blood.
Designing and conducting experiments to characterize in vitro models including assessments of in vitro potency cytotoxicity transcript/protein abundance using techniques such as viability assays microscopy histopathology and transcriptomics/proteomics.
Conducting experiments with a high level of independence and initiative and performing tasks with minimal direct supervision for routine methods.
Independently schedule and prioritize workload to manage several tasks simultaneously and identify / utilize resources outside of your own group.
Designing experiments to characterize models generating foundational datasets to guide model selection for specific drug program needs.
Maintaining detailed electronic documentation to ensure adherence to departmental procedures and contribute to final reports as needed.

Qualifications :

BS or MS degree (or equivalent) in cell or molecular biology bioengineering or a related field with typically 7 years (BS) or 5 years (MS) of experience.
Experience with standard cell and molecular biological assays such as ELISA/Luminex nucleic acid extraction qPCR immunofluorescence staining and confocal microscopy cell proliferation/viability assays cytotoxicity assays flow cytometry / FACS.
Experience with primary-derived cell culture differentiation methodologies; phenotypic and functional characterization of tissue-specific and/or immunological cells; understanding of key biomarkers for core tissue cell populations and/or tissue-resident or blood-derived immune cell populations.
Excellent organizational and analytical skills with the ability to independently troubleshoot optimize and innovate experimental approaches.
Excellent communication skills with an ability to work collaboratively in a matrixed research environment.

Preferred Qualifications:

Experience working with human tissue samples and with isolating and characterizing cells such as epithelial endothelial and/or immunological (B cells T cells monocytes).
Familiarity with automation or high-throughput screening if relevant as these are becoming increasingly important in advanced in vitro modeling.

Additional Information :

Applicable only to applicants applying to a position in any location with pay disclosure requirements under state or local law:

The compensation range described below is the range of possible base pay compensation that the Company believes in good faith it will pay for this role at the time of this posting based on the job grade for this position. Individual compensation paid within this range will depend on many factors including geographic location and we may ultimately pay more or less than the posted range. This range may be modified in the future.
We offer a comprehensive package of benefits including paid time off (vacation holidays sick) medical/dental/vision insurance and 401(k) to eligible employees.
This job is eligible to participate in our short-term incentive programs.

AbbVie is an equal opportunity employer and is committed to operating with integrity driving innovation transforming lives and serving our community. Equal Opportunity Employer/Veterans/Disabled.

US & Puerto Rico only - to learn more visit & Puerto Rico applicants seeking a reasonable accommodation click here to learn more:

Work :

Employment Type :

Full-time

Key Skills

Laboratory Experience
Immunoassays
Machine Learning
Biochemistry
Assays
Research Experience
Spectroscopy
Research & Development
cGMP
Cell Culture
Molecular Biology
Data Analysis Skills

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About Company

AbbVie

AbbVie is a global biopharmaceutical company focused on creating medicines and solutions that put impact first for patients, communities, and our world. We aim to address complex health issues and enhance people's lives through our core therapeutic areas: immunology, oncology, neuro ... View more

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