Biology Scientist II-Antibody Technologies (Req Exp in Flow Cytometry, FCS & Tissue Culture) Onsite

San Diego, CA - USA

Monthly Salary: Not Disclosed

Posted on: 18 hours ago

Vacancies: 1 Vacancy

Job Summary

Position : Biology Scientist II-Antibody Technologies

Location : San Diego CA

Duration : 12 Months

Total Hours/week : 40.00

1^st Shift

Client : Medical Devices Company

Level of Experience : Mid-Level

Employment Type : Contract on W2 (Need US Citizens or GC Holders or GC EAD or OPT or EAD or CPT

JOB DESCRIPTION

We are seeking a highly motivated and organized Scientist II to contribute to the research and development of monoclonal antibodies in our Antibody Technologies team.
This individual will be responsible for working in a dynamic team environment and be a contributor to multiple work streams including but not limited to protein expression and purification; tissue culture and cell-line engineering; and testing antibodies for use in flow cytometry application.
The position requires extensive hands-on experience with protein purification tissue culture protein expression and a strong understanding of biochemistry and molecular biology.
The successful candidate is expected to set up and execute experiments be detail-oriented skilled at data analysis and troubleshooting and will be accountable for preparing and presenting data at lab meetings with guidance regarding required tasks and expected results.

DUTIES AND RESPONSIBILITIES

Responsible for purification of antibodies and recombinant proteins using AKTA FPLC and manual methods. Must execute and document work using SOPs and must follow strict GMP requirements under ISO 13485 standards. Coordinates preparation and transfer of purified antibody and protein samples to other functional teams.
Executes analytical testing of purified antibodies and proteins using SEC SDS-PAGE Western blot MADLS and qPCR.
Responsible for transfection and/or expression of antibodies and recombinant proteins from various mammalian cell lines including but not limited to CHO and hybridomas as well as E. coli. Specific requirement for hands-on experience with aseptic technique and cell-culture techniques
Responsible for the execution of experiments with varying complexity using advanced molecular biology techniques.
Responsible for the execution of experiments with varying complexity using multi-color Flow Cytometry.
Analyze data using FCS Express and FlowJo software report test results including completion of all required documentation and database entries as required by protocols and SOPs.
Day-to-day problem solving and cross-functional team-based activities to ensure that daily operations and timelines are met.

Independently analyze own research data solve problems with minimal guidance and effectively plan follow up experiments. Will be expected to efficiently prioritize individual workload.
Involvement with scheduling operational and maintenance duties required within the laboratory.

KNOWLEDGE AND SKILLS

Protein purification with liquid chromatography and quality assurance using various analytical methods.
Protein expression using various systems including but not limited to mammalian cells insect cells and bacteria.
Proficient with aseptic technique and cell-culture techniques is required.
Preferred: Familiarity with assay automation and automated liquid handlers.
Preferred: Experience with cell line engineering
Preferred: Hand-on experience with Flow Cytometry and the ability of analyzing Flow data is preferred.

Effective interpersonal skills and ability to work in a cross-functional team-oriented environment to achieve organizational goals.

Effective written and oral communication skills.
Demonstrated ability to follow established policies and procedures.
Demonstrated ability to keep neat accurate and complete records.
Effective analytical and problem-solving skills.

EDUCATION AND EXPERIENCE

A Bachelors or Master of Science degree in biochemistry cellular or molecular biology immunology or related discipline and a minimum of 2-4 years of laboratory experience required. Protein purification protein expression molecular biology and tissue culture experience required.

Required Experience:

Position : Biology Scientist II-Antibody TechnologiesLocation : San Diego CADuration : 12 MonthsTotal Hours/week : 40.001st ShiftClient : Medical Devices CompanyLevel of Experience : Mid-LevelEmployment Type : Contract on W2 (Need US Citizens or GC Holders or GC EAD or OPT or EAD or CPTJOB DESCRIPTI...