Staff Scientist, Biochemistry, Karbstein
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Job Location:
Nashville, IN - USA
Monthly Salary:
Not Disclosed
Posted on:
Yesterday
Vacancies:
1 Vacancy
Job Summary
About the Work Unit:
The Karbstein Lab in the Department of Biochemistry at Vanderbilt University is seeking an enthusiastic RNA molecular biology/RNA biochemistry staff scientist to aid in the labs studies of ribosome assembly quality control and repair. In addition to providing a better understanding about the cellular mechanisms underlying ribosome integrity this basic science work is highly relevant to cancer biology and the biology of aging. The lab relies heavily on biochemistry and molecular biology as well as yest genetics. For examples of the type of research that will be undertaken see: Blomqvist et al 2023 PLoS Genetics; Yang et al 2023 Mol Cell; and Parker et al 2024 PLoS Biology; Yang&Karbstein BioRxiv 2024.
KeyFunctionsandExpectedPerformances:
Independently Carries out Research and Development:
Purifies ribosomes from various organisms for mass spectrometry analysis
Experience in RNA biochemistry is an asset
Uses protein gels Western blotting and mass spectrometry to analyze ribosome assembly factors and ribosomal proteins
Uses Northern blotting to analyze ribosome assembly in cells
Uses agarose gels to analyze PCR screens and PCR reactions
Generates yeast and worm strains and human cell lines
Uses quantitative growth measurements to monitor genetic interactions
Designs and clones proteins and RNAs for yeast worm or human in vivo work and in vitro assays
Analyzes RNAseq data
Analyzes experiments independently and prepares them for presentation to the PI
SupervisoryRelationships:
Might supervise interns work-study students or undergraduate researchers and explain problems to them.
The position reports administratively and functionally to the PI.
EducationandCertifications:
A PhD degree in cellular biology molecular biology biochemistry or a related field from an accredited institution of higher education and a minimum of two years of experience is preferred.
ExperienceandSkills:
Experience with protein and/or RNA biochemistry is strongly preferred.
Computational experience is preferred.
Being comfortable in learning new assays is necessary.
Independent design and execution of experiments is expected.
Ability to perform all basic calculations related to buffer composition (Molarity dilution factor etc.) is necessary.
Should be able to develop a short term vision for their project.
Communication skills are essential.
Required Experience:
Staff IC
Key Skills
- Laboratory Experience
- Bioinformatics
- Biochemistry
- Utility Locating
- Assays
- Research Experience
- Next Generation Sequencing
- Sensors
- Signal Processing
- Matlab
- Research & Development
- Molecular Biology
About Company
At Vanderbilt University , our work - regardless of title or role - is in service to an important and noble mission in which every member of our community serves in advancing knowledge and transforming lives on a daily basis. Located in Nashville , Tennessee, on a 330+ acre campus and ... View more
