qPCR Scientist III, Molecular Biology
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Job Location:
Monthly Salary:
Posted on:
3 days ago
Vacancies:
1 Vacancy
Job Summary
Work Schedule
Standard (Mon-Fri)Environmental Conditions
Cold Room/Freezers -22degreesF/-6degrees C Laboratory Setting Office Some degree of PPE (Personal Protective Equipment) required (safety glasses gowning gloves lab coat ear plugs etc.)Job Description
Key Responsibilities
Lead the design optimization and validation of qPCR assays for gene expression genotyping and molecular diagnostics applications.
Perform RNA/DNA extraction cDNA synthesis and nucleic acid quantification using spectrophotometric and fluorometric methods.
Validate assay performance including sensitivity specificity efficiency and reproducibility.
Analyze and interpret qPCR data using software such as Applied BiosystemsQuantStudio Bio-Rad CFX Manager or equivalent platforms.
Troubleshoot technical issues and propose innovative solutions to improve assay performance.
Maintainaccurateand detailed experimental records laboratory notebooks and data reports.
Collaborate cross-functionally with R&D QC and bioinformatics teams to support product development or experimental studies.
Ensure compliance with laboratory safety protocols quality management systems and regulatory standards (e.g. ISO GLP or CLIA as applicable).
Present experimental findings in technical meetings and contribute to scientific documentation and publications.
Mentor junior scientists and research associatesprovidingtechnical guidance and fostering a culture of collaboration and continuous improvement.
Qualifications
Education:Masters or Ph.D. in Molecular Biology Biochemistry Genetics ora relatedlife sciences discipline.Experience requirements will vary based ondegree.
Experience:
Minimum of 25 years of hands-on experience performing qPCR (probe basedchemistrypreferred) including multiplexed qPCR reactions.
Prior experience with assay development validation and data analysis.
Familiarity with nucleic acid extraction instrumentsor workflows is expected.
Additionalexperience in NGS is a plus.
Technical Skills:
In-depth understanding of molecular biology principles qPCR kinetics and assay troubleshooting.
Strong analytical and problem-solving skills with a data-driven mindset.
Strong understanding of qPCR principles primer/probe design andamplificationkinetics.
Proficiencyin data analysis statistical interpretation and report generation.
Experience working under quality and regulatory standards preferred.
Soft Skills:
Excellent organizational communication and problem-solving skills.
Ability to work independently as well as collaboratively in a multidisciplinary environment.
Strong attention to detail and scientific curiosity.
Preferred Qualifications
Familiarity with bioinformatics tools forassay design
Experience withdigital PCR (dPCR)
Knowledge of automation and data management systems.
Required Experience:
IC
Key Skills
- Laboratory Experience
- Immunoassays
- Machine Learning
- Biochemistry
- Assays
- Research Experience
- Spectroscopy
- Research & Development
- cGMP
- Cell Culture
- Molecular Biology
- Data Analysis Skills
About Company
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