qPCR Molecular Biologist, Scientist II
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Job Location:
Monthly Salary:
Posted on:
2 days ago
Vacancies:
1 Vacancy
Job Summary
Work Schedule
Standard (Mon-Fri)Environmental Conditions
Cold Room/Freezers -22degreesF/-6degrees C Laboratory Setting Office Some degree of PPE (Personal Protective Equipment) required (safety glasses gowning gloves lab coat ear plugs etc.)Job Description
Key Responsibilities
Design develop andoptimizeqPCR assays for gene expression genotyping copy number variation and pathogen detection.
Plan and execute experiments independently ensuring data quality reproducibility and technical excellence.
Perform nucleic acid extraction cDNA synthesis and quantification using standard molecular methods.
Validate assay performance (sensitivity specificity and efficiency) and analyze results using platforms such as Applied BiosystemsQuantStudio or Bio-Rad CFX Manager.
Troubleshoot technical issues and implement improvements to enhance assay performance.
Maintain detailed experimental records and prepare technical reports SOPs and validation documentation.
Collaborate with product development bioinformatics and assay design teams to integrate molecular workflows.
Ensure adherence to safety protocols quality systems and regulatory requirements (ISO GLP CLIA).
Qualifications
Education:Bachelors orMasters in Molecular Biology Biochemistry Genetics ora relatedlife sciences discipline.Experiencerequirementswill vary based ondegree.
Experience:
Minimum of1-4years of hands-on experience performingqPCR.Experience requirements will vary based ondegree.
Prior experience with assay development validation and data analysis.
Familiarity with nucleic acid extraction instruments is a plus.
Technical Skills:
Understanding of molecular biology principles qPCR kinetics and assay troubleshooting.
Strong analytical and problem-solving skills with a data-driven mindset.
Strong understanding of qPCR principles primer/probe design andamplificationkinetics.
Proficiencyin data analysis statistical interpretation and report generation.
Experience working under quality and regulatory standards preferred.
Soft Skills:
Excellent organizational communication and problem-solving skills.
Ability to work independently as well as collaboratively in a multidisciplinary environment.
Strong attention to detail and scientific curiosity.
Preferred Qualifications
Familiarity with bioinformatics tools forassay design
Experience withNGS
Experience withdigital PCR (dPCR)
Experience with qPCR multiplexing
Knowledge of automation and data management systems.
Key Skills
- Laboratory Experience
- Transfection
- Bioinformatics
- DNA Isolation
- Genetics
- PCR
- R
- Biochemistry
- Next Generation Sequencing
- Cell Culture
- Molecular Biology
- Research Laboratory Experience
About Company
Electron microscopes reveal hidden wonders that are smaller than the human eye can see. They fire electrons and create images, magnifying micrometer and nanometer structures by up to ten million times, providing a spectacular level of detail, even allowing researchers to view single a ... View more
