Research Associate Microbiology & Immunology (Dudek Lab)
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Job Location:
Monthly Salary:
Posted on:
19 hours ago
Vacancies:
1 Vacancy
Job Summary
This Research Associate position in Dr. Dudeks laboratory will conduct research focused on the pathogenesis and immune evasion of HIV-1. Specifically this position will use gene editing of primary immune cells including T cells Natural Killer cells B cells and hematopoietic stem and progenitor cells followed by ex-vivo differentiation and infection to investigate genes altering replication and immune recognition of infection. This position will require development of new techniques write up of protocols execution of experiments data analysis and manuscript writing. The candidate will also be required to mentor undergraduate students summer students and rotating graduate students. The position will require work in a BSL2 laboratory.
Position Responsibilities
Perform critical analyses of literature relevant to research undertaken.
Interpret and adapt published protocols to specific experiments.
Develop new hypotheses.
Help mentor students and collaborators.
Perform experimental procedures; record and consolidate data.
Complete work within specified time guidelines; discuss progress and any problems as needed.
Maintain and passage multiple cell lines.
PBMC isolation from whole blood.
Maintain and differentiate primary immune cells.
Produce and purify AAV and HIV-1 from transfected cells.
Use digital droplet PCR to quantify viral stocks infection rate and editing efficiency.
Perform molecular biology experiments including cloning construction of vectors for viral (AAV and HIV-1) production and make mutants using site-directed mutagenesis.
Prepare chemical solutions and media according to established protocols.
Maintain primary cell and cell line stocks and inventory.
Conduct independent research to identify and characterize cellular genes altering HIV-1 infectivity.
Prepare figures and write manuscripts for publication.
Edit grant proposals for principal investigator.
Edit paper submissions for students and other lab personnel.
Coordinate safety program with EHS and lab members. Document safety training.
Reconcile lab procurement card and monthly grant reports. Assure charges are valid and resolve errors.
Assist in maintaining accurate records of laboratory databases (equipment and supplies reagents microbial strains and stocks and animals).
Maintain laboratory supplies and equipment including the timely ordering receiving and proper storage of supplies and equipment.
Assist in maintaining general laboratory functionality.
Assist in developing new protocols.
Maintain a clean work area.
Train lab personnel on protocols.
Provide feedback to new and current employees.
Help mentor students and collaborators.
Oversee undergraduate and graduate students in successful completion of assigned tasks and research.
Percent of Time: 100%
Staff Type: Professional & Scientific
Type of Position: Specified Term. Initial appointment is for one year. Appointment may be extended based on performance and availability of funding.
Pay Grade: 4A - Highlights:
Regular salaried position located in Iowa City Iowa
Fringe benefit package including paid vacation; sick leave; health dental life and disability insurance options; and generous employer contributions into retirement plans
For more information about Why Iowa clickhere
Education Requirements
- Requires a bachelors degree in Microbiology biology Cell Biology or Immunology or a related field.
Prior experience working with pathogenic microorganisms is required.
Excellent written and verbal communication skills.
Excellent organizational skills.
Three or more years of lab experience working in a microbiology cell biology immunology or biochemistry laboratory including:
- Experience with standard virology and viral vector techniques including virus production by transient transfection ultracentrifugation and MOI calculation.
- Experience working in a BSL2 laboratory.
- Experience with primary immune cells.
- Experience with protein purification SDS-PAGE and Western analysis.
- Molecular biology experience including PCR primer design gene cloning plasmid and PCR purification agarose gel electrophoresis and restriction enzyme analyses.
- Using fluorescence microscopy to check infectivity and editing rates
- Use flow cytometry to characterize immune marker expression during and after infection and quantify HIV-1 latency
- Gathering recording and analyzing of experimental data.
- Data analysis and presentation software such as Microsoft Excel Prism Microsoft Word and Photoshop.
- Proficiency with each of the following:
- Ability to effectively prioritize projects to consistently meet deadlines.
- Ability to multitask in a fast-paced environment.
- Attention to detail and accuracy.
- Ability to work in a team.
Desired Qualifications
Extensive experience with cell culture.
Prior experience with Immunofluorescence microscopy and flow cytometry
Prior experience with infections pathogens such as HIV-1.
Application Process: In order to be considered applicants must upload a CV or resume and cover letter (under submission relevant materials) that clearly address how they meet the listed required and desired qualifications of this position.
Job openings are posted for a minimum of 7 calendar days and may be removed from posting and filled any time after the original posting period has ended. Successful candidates will be required to self-disclose any conviction history and will be subject to a criminal background check. This position is not eligible for University sponsorship for employment authorization.
For additional questions please contact Anne Beyerink at
Required Experience:
IC
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About Company
The University of Iowa is a top-tier public research university in Iowa City—home to more than 32,000 students, offering world-class programs across 200+ fields of study, renowned especially for its creative writing, acclaimed medical center, and balanced excellence in the arts and sc ... View more
