Entry Laboratory Sciences Professional – Appel Research Lab

Job Summary:

A position as an Entry Laboratory Sciences Professional is available within the Appel research laboratory in the Department of Pediatrics Section of Developmental Biology at the University of Colorado Anschutz. This is a full-time (1.0FTE) position that will utilize molecular biochemical genetic light microscopy and zebrafish embryological approaches in a wet lab setting.

The Appel lab studies the formation of neurons and glial cells in the developing nervous system. As an Entry Laboratory Sciences Professional in our lab you will work closely with graduate students and postdoctoral fellows to support their projects. The work is highly flexible and variable and includes standard molecular biology techniques (PCR subcloning gel electrophoresis in situ RNA hybridization immunohistochemistry) light microscopy (fluorescence and confocal microscopy) zebrafish husbandry and zebrafish embryological experiments.

Key Responsibilities:

• Collaborate with and support Principal Investigators (PI) and other stakeholders with laboratory and research functions

• Utilize a variety of laboratory techniques required for research projects

• Perform experiments tabulate and analyze research

• Support analytical testing process using standard lab support knowledge in the collection and review of conditions including preserving samples completing sample preparation documenting and communicating results

• Perform pre-analytical analytical and post-analytical test processes and evaluate the results produced

• Prepare lab for daily operations including stocking materials equipment safety cleanliness and related areas

• Provide animal care related to research studies as necessary

• Pick up transport and maintain results samples documents materials and other items as needed

• Dispose of laboratory materials and samples using well-established guidelines and instructions

• Review complete and process logs documents databases and related materials and information

• Provide support in researching issues and developing recommendations to resolve process unit and related issues

• Assist in the review and implementation of lab support policies practices and programs to meet organizational operational and management needs

• Assist with ensuring compliance with applicable laws rules and regulations; receive guidance and direction or escalate when required

Additional Duties & Responsibilities:

• Genotype zebrafish embryos larvae and adults

• Perform molecular cloning PCR and gel electrophoresis

• Perform microinjections into zebrafish eggs for transgenesis and CRISPR/Cas9 experiments

• Perform in situ RNA hybridization and immunohistochemistry

• Analyze samples using confocal light microscopy

Work Location:

Why Join Us:

Onsite this role is expected to work onsite and is located in Aurora CO.

The Appel research group is part of a highly cohesive academic unit in the Section of Developmental Biology within the Department of Pediatrics. The Section of Developmental Biology is committed to research that improves child health with five main objectives:

• Understand the genetic molecular and cellular mechanisms that guide normal development

• Determine how normal developmental processes are altered to result in developmental disease and disability

• Identify new diagnostic strategies and molecular and cellular therapies targeted at the treatment of childhood disease

• Train the next generation of leaders in developmental and stem cell biology and pediatric disease research

• Serve as a hub for basic and translational child health research that spans the Anschutz Medical Campus research community

To accomplish these objectives the faculty graduate students postdoctoral trainees and research staff who comprise the Section of Developmental Biology perform research using stem cell organoid and animal experimental models including Drosophila zebrafish and mice. Our investigations focus on several major tissue and organ systems such as the brain heart intestine face vascular olfactory and immune systems. Our work is leading to a better understanding of childhood disabilities associated with neuropsychiatric disease Down syndrome the congenital basis of cardiovascular and facial malformations and the impact of maternal health on fetal development.

Qualifications:

Minimum Qualifications:

• Bachelors degree in biology molecular biology chemistry genetics or related field

Applicants must meet minimum qualifications at the time of hire.

Preferred Qualifications:

• Masters degree in biology molecular biology chemistry genetics or related field

• Prior experience or background within an experimental or wet lab

Knowledge Skills and Abilities:

• Knowledge of basic laboratory techniques including safety procedures and techniques

• Ability to communicate effectively both in writing and orally

• Ability to establish and maintain effective working relationships with employees at all levels throughout the institution

• Outstanding customer service skills

• Knowledge of basic human anatomy physiology and medical terminology

• Ability to interpret and master complex research protocol information

How to Apply:

For full consideration please submit the following document(s):

1. A letter of interest describing relevant job experiences as they relate to the listed job qualifications and interest in the position

2. Curriculum vitae / Resume

3. Three to five professional references including name address phone number (mobile number if appropriate) and email address

Questions should be directed to: Bruce Appel

Screening of Applications Begins:

Immediately and continues until the position is filled. For best consideration apply by December 15 2025.

Anticipated Pay Range:

The starting salary range (or hiring range) for this position has been established as

$46274 to $58861.

The above salary range (or hiring range) represents the Universitys good faith and reasonable estimate of the range of possible compensation at the time of posting. This position is not eligible for overtime compensation unless it is non-exempt.

Your total compensation goes beyond the number on your paycheck. The University of Colorado provides generous leave health plans and retirement contributions that add to your bottom line.

Total Compensation Calculator:

Equal Employment Opportunity Statement:

The University of Colorado (CU) is an Equal Opportunity Employer and complies with all applicable federal state and local laws governing non-discrimination in employment. We are committed to creating a workplacewhere all individuals are treated with respect and dignity andwe encourage individuals from all backgrounds to apply including protected veterans and individuals with disabilities.

ADA Statement:

The University will provide reasonable accommodations to applicants with disabilities throughout the employment application process. To request an accommodation pursuant to the Americans with Disabilities Act please contact the Human Resources ADA Coordinator at .

Background Check Statement:

The University of Colorado Anschutz Medical Campus is dedicated to ensuring a safe and secure environment for our faculty staff students and visitors. To assist in achieving that goal we conduct background investigations for all prospective employees.

Vaccination Statement:

CU Anschutz strongly encourages vaccination against the COVID-19 virus and othervaccine-preventable diseases. If you work visit or volunteer in healthcare facilities or clinics operated by our affiliated hospital or clinical partners or by CU Anschutz you will be required to comply with the vaccination and medical surveillance policies of the facilities or clinics where you work visit or volunteer addition if you work in certain research areas or perform certain safety-sensitive job duties you must enroll in theoccupational health medical surveillance program.