Postdoctoral Fellow-MSH
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Job Location:
New York City, NY - USA
Monthly Salary:
Not Disclosed
Posted on:
3 hours ago
Vacancies:
1 Vacancy
Job Summary
Salary: $74692
Department: Oncological Sciences
Physical work location:
Icahn School of Medicine at Mount Sinai
1468 Madison Ave
Annenberg 24-60
New York NY 10029
Name PI: Elvin Wagenblast PhD
Web link to Lab:
Responsibilities
Details of Research Project:
The Wagenblast Lab investigates how somatic evolution in the hematopoietic system influences cancer progression. The postdoctoral fellow will focus on a project exploring how TET2-mutant clonal hematopoiesis remodels the bone marrow into a metastasis-permissive niche for solid tumors particularly breast cancer. Using humanized xenograft models the lab studies the inflammatory and stromal alterations induced by clonal hematopoiesis and evaluates therapeutic strategies targeting cytokine signaling. The fellow will leverage cutting-edge tools including CRISPR/Cas9 genome editing single-cell and spatial transcriptomics and preclinical mouse modeling to dissect how mutant immune cells orchestrate metastatic progression.
Technical Duties: (include any protocols)
- Generate genome-edited hematopoietic stem/progenitor cells (HSPCs) using CRISPR/Cas9
- Perform xenotransplantation of human HSPCs into immunodeficient mice
- Analyze bone marrow and tumor samples via flow cytometry immunohistochemistry and histology
- Conduct bioluminescence imaging to monitor metastasis progression
- Assist in spatial transcriptomics using 10x Visium HD and single-cell RNA sequencing workflows
- Maintain primary cell cultures perform viral transduction and cytokine assays
- Participate in manuscript preparation and presentation of findings
Qualifications
Educational and other Requirements for the position:
- PhD in cancer biology hematology immunology or a related biomedical discipline
- Demonstrated experience in molecular biology and murine models
- Strong communication skills and ability to work independently and collaboratively in a team
Experience Required:
- Prior experience with CRISPR-based genome editing and/or xenograft models
- Familiarity with hematopoietic stem cell biology and immune phenotyping preferred
- Experience analyzing high-dimensional datasets (e.g. scRNA-seq) is an asset but not required
Goals/Outcomes of the Research Project:
- Define the molecular and cellular mechanisms by which mutant clonal hematopoiesis alters the bone marrow niche
- Identify specific cytokines and cell types responsible for metastasis promotion
- Evaluate targeted interventions in preclinical models
- Generate high-impact publications and lay the foundation for future host-targeted metastasis prevention strategies
Key Skills
- Virology
- Bioinformatics
- Genetics
- R
- Biochemistry
- Cell Biology
- Research Experience
- Spectroscopy
- Cell Culture
- Molecular Biology
- Microscopy
- Research Laboratory Experience
About Company
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