Temp Scientist I, Protein Chemistry
Temp Scientist I, Protein Chemistry
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Salary Not Disclosed
1 Vacancy
Job Description
Revolution Medicines is a clinical-stage precision oncology company focused on developing novel targeted therapies to inhibit frontier targets in RAS-addicted cancers. The companys R&D pipeline comprises RAS(ON) Inhibitors designed to suppress diverse oncogenic variants of RAS proteins and RAS Companion Inhibitors for use in combination treatment strategies. As a new member of the Revolution Medicines team you will join other outstanding Revolutionariesin a tireless commitment to patients with cancers harboring mutations in the RAS signaling pathway.
The Opportunity:
We are seeking an experienced and creative protein biochemist to support our in-house protein chemistry team within the Structural Biology group. They will be responsible for purifying and characterizing small molecule protein targets to support screening pipelines biophysical studies and structural biology. The ideal candidate will have a strong track record of leveraging internal and external resources to produce high quality proteins of therapeutic interest to support downstream in vitro assays and structural biology efforts. This role will report to the principal scientist leading protein chemistry and will have responsibility and visibility across the early discovery pipeline. This is an excellent opportunity for an experienced individual looking to work in a fast-paced dynamic biotech environment. The candidate will have a range of responsibilities detailed below.
Expertise in all steps of the gene-to-protein process. Experienced in generation of high-quality recombinant proteins that are well characterized using biophysical and biochemical techniques.
Perform expression purification (with and without affinity tags) and characterization of challenging targets to establish robust protocols that can be used internally or transferred to external (CRO) resources as needed. Ability to evaluate and establish protocols transferred in from external resources.
Develop robust and reproducible biochemical and biophysical assays to characterize enzymatic activity protein stability protein-protein interaction etc.
Support protein chemistry by exploring new technologies contribute to long-term activity planning and budgets and maintaining modern instrumentation in the laboratory.
Communicate experimental design results and timelines to cross-functional teams
Generate high-quality reproducible data. Analyze and interpret data keep organized and detailed records. Communicate findings to cross functional team members.
Partner and integrate with multidisciplinary highly collaborative discovery teams to design optimize and produce reagents that play a critical role in structure-based drug design and screening
Required Skills Experience and Education:
Ph.D. degree MS 5 BS 7 years experience (or equivalent) in biochemistry structural biology or related discipline biopharmaceutical experience is a plus.
Expertise with expression of proteins and protein complexes using multiple host systems (e.g. mammalian insect bacterial).
Expert in designing and optimizing DNA constructs for expression and purification of challenging targets.
Expert in protein purification using AKTA and multiple chromatography techniques (e.g. affinity IEX SEC HIC).
Extensive protein biochemistry experience working with challenging proteins and multi-protein complexes.
Knowledge of protein structure and experience in the preparation of high-quality protein samples for assay (e.g. BLI SPR DEL TR-FRET) biophysical (e.g. ITC DSF MALS SLS DLS CD anSEC thermal melt LC-MS) and structural studies (e.g. NMR X-ray crystallography Cryo-EM).
Creative and capable of developing new techniques and methods to drive science forward.
Excellent written and verbal communication skills.
Ability to develop and optimize written protocols and document research progress.
Conscientious team-player interested in a fast-paced biotech environment.
Demonstrated willingness to learn and improve processes.
Preferred Skills:
Experience with mass spectrometry and sample preparation is desired.
Experience balancing CRO and internal resources to solve problems and drive progress is beneficial.
Clear examples of establishing new technologies to increase throughput in protein expression/purification.
Demonstrated collaborations with Structural Biology and/or Assay groups through publications and/or patents.
Experience with protein-protein complexes including antibodies/Fabs molecular glues PROTACs etc. highly desirable.
This is a contract position that will be employed through a third-party recruiting agency. The agency will provide the pay range which will be based on several factors including job-related skills experience market conditions and relevant education or training.
Revolution Medicines is an equal opportunity employer and prohibits unlawful discrimination based on race color religion gender sexual orientation gender identity/expression national origin/ancestry age disability marital status medical condition and veteran status.
Revolution Medicines takes the protection and security of personal data very seriously and respects your right to privacy while using our website and when contacting us by email or phone. We will only collect process and use any personal data that you provide to us in accordance with ourCCPA additional information please contact.
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Employment Type
Temp
