Postdoctoral Research Associate â Structural Biology or Cell Biology â Fontana Lab
Postdoctoral Research Associate â Structural Biology or Cell Biology â Fontana Lab
Employer Active
Job Alert
You will be updated with latest job alerts via email
Share
Job Alert
You will be updated with latest job alerts via email
Not Disclosed
Salary Not Disclosed
1 Vacancy
Job Description
Project Description:
Dr. Pietro Fontana Assistant Member in the Center of Excellence for Pediatric Immuno-Oncology (CEPIO) and Department of Immunology is seeking passionate and highly motivated candidates for a fully funded postdoctoral fellowship in either Structural Biology or Cell Biology. Candidates with specialty in either field are encouraged to apply. Please include a cover letter describing your interest.
The Fontanalab focuses on understanding how immune receptors recognize danger signals and initiate downstream responses with a particular emphasis on the mechanistic basis of activation and small molecule modulation.We investigate pathways involving cytosolic DNA sensors inflammasome components and adaptor proteins such as cGAS AIM2 and TLRs. Our research integrates cryo-EM cell biology and chemical biology to dissect how molecular architecture governs immune function. Recent work includes the discovery and structural characterization of novel dsDNA sensors and the identification of small molecules targeting key innate immune components Responsibilities:
Option 1: Structural Biology
Express and purify immune signaling proteins and complexes using bacterial insect and mammalian systems.
Prepare samples for structural studies by cryo-electron microscopy (cryo-EM) and/or X-ray crystallography.
Perform data processing and model building (e.g. Relion CryoSPARC Coot PyMOL).
Option 2: Cell Biology
Design and execute cell-based assays to investigate innate immune signaling pathways including interferon responses NfKb pathway and cell death.
Engineer and characterize genetic models including CRISPR/Cas9 knockouts and inducible systems in mammalian cells.
Analyze immune pathway activation using molecular biology techniques (qPCR Western blotting ELISA flow cytometry) and microscopy.
Additional training in immunology structural biology biochemistry and cell biology will be provided for highly motivated candidates.
Minimum Education and/or Training:
Successful applicants will hold a Ph.D. in cell biology structural biology or related discipline. The candidate should demonstrate an academic record of excellence and a strong interest in the growing field of structural immunology.
Special Skills Knowledge and Abilities:
Option 1: Structural Biology
Experience with protein expression purification and biochemical characterization using bacterial insect or mammalian cell culture.
Good working knowledge of single particle cryo-EM pipeline and data processing.
Prior knowledge of basic cell culture and cell-based assays
Option 2: Cell Biology
Experience with mammalian cell culture gene editing (CRISPR/Cas9) and generation of stable cell lines (lentivirus).
Strong background in cell signaling innate immunity or related areas.
Proficiency in molecular biology and immunoassays (qPCR Western blotting ELISA flow cytometry reporter assays).
Experience with fluorescence microscopy and imaging-based assays.
Excellent communication and presentation skills and contribute to preparation of high-quality manuscripts.
About the Lab and Institution:
The Fontana Lab fosters a collaborative and dynamic research environment deeply committed to the professional development of its members. We value ideas and love for science and encourage active participation in growing opportunities such as grant writing attendance at immersive workshops and presenting research at internal and external meetings.
St. Jude Childrens Research Hospital is a world-class research institution focused on groundbreaking pediatric cancer research. With access to state-of-the-art core facilities cutting-edge technologies and a vibrant scientific community we provide an ideal environment for postdoctoral researchers to thrive and make significant contributions to the field of cancer immunotherapy.
Why Join Us
State-of-the-Art Resources: Access to institutional core facilities equipped with advanced technologies and led by expert scientists.
Tailored Career Development: Personalized programs designed to enhance professional growth including grant writing workshops mentorship opportunities and more.
Competitive Compensation: Starting salary of $72530 per year plus comprehensive benefits and relocation assistance.
No Search Firms
St. Jude Childrens Research Hospital does not accept unsolicited assistance from search firms for employment opportunities. Please do not call or email. All resumes submitted by search firms to any employee or other representative at St. Jude via email the internet or in any form and/or method without a valid written search agreement in place and approved by HR will result in no fee being paid in the event the candidate is hired by St. Jude.
Required Experience:
IC
Employment Type
Full-Time
