Senior Lab Technician RESEARCH NEUROLOGY V Lab

Site: The Brigham and Womens Hospital Inc.

Mass General Brigham relies on a wide range of professionals including doctors nurses business people tech experts researchers and systems analysts to advance our mission. As a not-for-profit we support patient care research teaching and community service striving to provide exceptional care. We believe that high-performing teams drive groundbreaking medical discoveries and invite all applicants to join us and experience what it means to be part of Mass General Brigham.

New budgeted research role

Job Summary

Summary
Carries out a broad range of technically advanced research activities and procedures; evaluates conclusions and has considerable latitude to modify or devise methods and techniques as necessary to achieve desired results; typically supervises technical and sometimes administrative elements of the research unit. Helps onboard new staff and train them in the lab.

Essential Functions
-Develops or provides input into modifications of existing assay techniques experimental protocols or new procedures.
-Synthesizes and interprets results.
-Prepares written and/or verbal status reports.
-Performs statistical analysis and makes recommendations.
-Independently performs non-routine highly specialized experimental procedures.
-Composes and may present sections of research reports and manuscripts.

In vivo Research Support 80%

Collaborate closely with lab members to generate high-quality data for publications grant proposals and presentations.
Assist in the design and execution of experiments related to peripheral nervous system and peripheral cancers
Perform precise dissections of peripheral nerve tissues from animal models
Assist in advanced microscopy imaging including confocal and fluorescence microscopy and take ownership of image acquisition processing and quantitative analysis.
Perform mouse in vivo animal procedures including dosing animals by various routes (e.g. oral intravenous intraperitoneal etc.) measuring tumors by calipers non-invasive imaging (bioluminescent imaging) and breeding of genetically engineered mouse models.
Optimize and troubleshoot immunofluorescence immunohistochemistry and in situ hybridization protocols.
Mentor and train new lab members and junior staff in specialized techniques such as mouse handling genotyping and experimental design.

Cell Culture 10%

Differentiate expand and maintain multiple iPSC lines ensuring cell quality and reproducibility across experiments.
Perform advanced mammalian cell culture techniques including routine maintenance transfection and differentiation of various cell lines.
Develop and maintain primary cultures from animal tissue including neurons and glial cells.

Lab Upkeep 10%

Maintain a clean and well-organized work environment ensuring proper storage of reagents cells and samples.
Manage laboratory inventories including ordering reagents and maintaining stock levels of commonly used consumables.
Calibrate and perform routine maintenance on critical lab equipment such as microscopes centrifuges and PCR machines.
Assist in ensuring compliance with institutional biosafety animal care and chemical safety regulations.
Help maintain accurate lab documentation including protocols experimental records and safety data sheets.

Education: MSc preferred bachelors degree in biology or related major required. 3-5 years of relevant laboratory experience outside coursework required.

• Demonstrated experience with mammalian tissue culture and aseptic technique.
• Experience in rodent handling dosing such as intravenous (IV) intraperitoneal (IP) intramuscular (IM) or subcutaneous (SC) and tissue collection
• Must have demonstrated experience in murine survival surgery and necropsy.
• Experience with small animal imaging modalities a plus
• Ability to design execute and troubleshoot protocols and experiments for various projects in the lab.
• Must be able to maintain detailed and accurate laboratory notebooks documenting experimental procedures results and observations.
• Compile analyze and present data in a timely fashion.

Knowledge Skills and Abilities
- Excellent communication skills.
- Good interpersonal skills and the ability to oversee the work of others.
- Ability to work independently.
- Ability to identify problems and develop solutions.
- Ability to prioritize tasks and set deadlines.
- Strong database management and overall computer skills.
- Functions with speed precision and an understanding of theory behind tests and procedures.

Onsite

60 Fenwood Road

40

Regular

Day (United States of America)

The Brigham and Womens Hospital Inc. is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race color religious creed national origin sex age gender identity disability sexual orientation military service genetic information and/or other status protected under law. We will ensure that all individuals with a disability are provided a reasonable accommodation to participate in the job application or interview process to perform essential job functions and to receive other benefits and privileges of employment. To ensure reasonable accommodation for individuals protected by Section 503 of the Rehabilitation Act of 1973 the Vietnam Veterans Readjustment Act of 1974 and Title I of the Americans with Disabilities Act of 1990 applicants who require accommodation in the job application process may contact Human Resources at .

Mass General Brigham Competency Framework