Postdoctoral Researcher
Postdoctoral Researcher
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Job Description
Union: Non-Union
Number of vacancies: 1
Site: McEwen Stem Cell Institute at the University Health Network
Department: McEwen Stem Cell Institute
Reports to: Principal Investigator
Hours: 37.5 hours per week
Shifts: Monday to Friday
Status: Temporary Full-Time
Closing Date: June 27 2025
Who we are
What we offer
- A welcoming dynamic international team with cross-disciplinary expertise in pluripotent stem cell biology developmental biology molecular genetics cardiac surgery and electrophysiology
- The opportunity to work at the forefront of stem cell research with a focus towards clinical translation
- The opportunity to interact with a wide network of international collaborators
- The opportunity to join Torontos vibrant research community
What we are looking for
A driven motivated postdoctoral fellow for a project focused on human pluripotent stem cell-derived Atrioventricular Node-Like Pacemaker Cells (AVNLPCs) and their application as a biological conduction bridge. The start date for this position is flexible and preferably summer/fall 2025.
The postdoctoral fellow will:
- Build on a previously established directed differentiation protocol for AVNLPCs
- Assess how different cardiogenic mesoderm origins impact the phenotype of AVNLPCs
- Molecularly analyze the AVNLPCs and benchmark them to human AVN tissue using single-nuclei RNA sequencing
- Functionally analyze the AVNLPCs by embedding them into engineered heart tissues and using optical mapping
- Scale up the established AVNLPC protocol using vertical wheel bioreactors
- Collaborate with experienced cardiovascular surgeons to establish a pig model of AV-block and test the ability of AVNLPCs to function as a biological conduction bridge.
- Coordinate a team of veterinary technicians and surgeons to accomplish the pig experiments.
- Analyze telemetric ECG recordings from the pig experiments
- Perform immunohistological analysis of the cell grafts in the pig hearts.
Qualifications :
- Received a PHD or MD degree within the past 5 years.
- Experience with tissue culture techniques
- Experience in flow cytometry
- Experience working with animal models
- Experience in immunohistochemistry
- Experience in molecular biology techniques (RT-qPCR cloning western blot)
- Effective organizational time management and prioritization skills
- Strong problem recognition and problem-solving skills
- Strong verbal and written communications skills
- Ability to effectively work as part of a team
The following would be considered as valuable assets:
- Background in cardiovascular research
- Experience culturing and differentiating human pluripotent stem cells
- Experience in cardiac differentiations of human pluripotent stem cells
- Experience working with cardiac animal models
- Experience with cell engraftment into animal models
- Experience in optical mapping techniques
- Experience with computational analysis of single-cell RNA sequencing experiments
Additional Information :
Why join UHN
In addition to working alongside some of the most talented and inspiring healthcare professionals in the world UHN offers a wide range of benefits programs and perks. It is the comprehensiveness of these offerings that makes it a differentiating factor allowing you to find value where it matters most to you now and throughout your career at UHN.
- Competitive offer packages
- Government organization and a member of the Healthcare of Ontario Pension Plan (HOOPP access to Transit and UHN shuttle service
- A flexible work environment
- Opportunities for development and promotions within a large organization
- Additional perks (multiple corporate discounts including: travel restaurants parking phone plans auto insurance discounts on-site gyms etc.)
Current UHN employees must have successfully completed their probationary period have a good employee record along with satisfactory attendance in accordance with UHNs attendance management program to be eligible for consideration.
All applications must be submitted before the posting close date.
UHN uses email to communicate with selected candidates. Please ensure you check your email regularly.
Please be advised that a Criminal Record Check may be required of the successful candidate. Should it be determined that any information provided by a candidate be misleading inaccurate or incorrect UHN reserves the right to discontinue with the consideration of their application.
UHN is an equal opportunity employer committed to an inclusive recruitment process and workplace. Requests for accommodation can be made at any stage of the recruitment process. Applicants need to make their requirements known.
We thank all applicants for their interest however only those selected for further consideration will be contacted.
Remote Work :
No
Employment Type :
Contract
Employment Type
Contract
