Protein Science Senior Research Scientist Purification and Analytics - Contract
Protein Science Senior Research Scientist Purification and Analytics - Contract
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Job Description
Job Description
Protein Science Senior Research Scientist (Purification and Analytics)
General/Position Summary
The Protein Sciences Senior Research Scientist is an expert in purifying (mg to grams scale) and characterizing therapeutic proteins and reagents for earlystage and latestage discovery research programs. The Senior Research Scientist is a subject matter expert for protein purification and protein analytics for crossfunctional project teams. The Senior Research Scientist is seeking opportunities to improve upon existing platform processes by bringing in new technologies in automation and instrumentation along with their deep knowledge in protein purification and characterization.
Key Duties & Responsibilities
- Operate the AKTA Avant and AKTA Explorer systems (Unicorn software) to purify antibodies Fc fusions and histidinetagged protein reagents suitable for both invitro and invivo testing
- Design a purification strategy utilizing various techniques including ProA IMAC Ion Exchange HIC and Preparative SEC based on a fitforpurpose approach
- Generate analytical characterization data using UVVis Spectroscopy analytical SEC (UPLCHPLC through Empower software) endotoxin tests SDSPAGE and western blotting
- Engage in collaborations across crossfunctional teams and with vendors for mass spectroscopy analysis
- Optimize BLI assays to determine kinetics of therapeutic proteins for proteinprotein interaction (KD koff kon
- Deliver verbal and presentation updates on purification and analytics to the Protein Science Team Project Team and a broader Research Team
- Ensure that a complete and timely record of protein lots is maintained in both the protein database and electronic lab notebooks
Required Education Level
- PhD. (or equivalent degree) in cellular biology biochemistry biophysics or bio/chemical engineering and 3 years of relevant industry experience or
- Masters Degree (or equivalent degree) in cellular biology biochemistry biophysics or bio/chemical engineering and 6 years of relevant industry experience or
- Bachelors Degree (or equivalent degree) in cellular biology biochemistry biophysics or bio/chemical engineering and 10 years of relevant industry experience
Required Knowledge/Skills
- Proven expertise in utilizing Unicorn software and AKTA systems for protein purification along with a solid understanding of related instrumentation
- Extensive handson experience in various protein capture and polishing techniques using resins like MabSelect SuRE MabSelect PrismA Capto L Capto DEAE Capto Q and Capto S
- Proficient in using Empower software to execute SEC HIC and reversed phase methods with Waters HPLC and UPLC systems along with familiarity with the required instrumentation
- Experience in running SDSPAGE and developing protocols for Western blot analysis
- Experience in running kinetics or quantitative assays using BLI systems such as Gator Bio or Forte Octet
- Experience in ranking variants based on protein stability and manufacturability
- Familiarity with Mass Spectrometry for identification thermal melt analysis for protein stability
- Knowledge of running cIEF assays using PA800 (Sciex) system to analyze charged variants is a plus
- Experience in working in a highly collaborative laboratory which is timelinedriven and result orientated team is a must
- Experience in mammalian cell culture work to transiently express recombinant proteins is plus
- Experience in working on earlydiscovery protein therapeutic candidates that matured into clinical development is a plus
Location:
188 East Blaine Street
Suite 200
Seattle Wash. 98102
Pay Range
Dependent upon experience $50$60/HR
Requisition Disclaimer
This job posting is for a temporary role with a thirdparty agency partner on assignment at Vertex Pharmaceuticals (Vertex). The individual selected for this role will be offered the role as an employee of a third party agency partner; compensation medical benefits fringe benefits and other terms and conditions of employment shall be presented by the thirdparty agency partner upon offer. The pay rate range provided is a reasonable estimate of the anticipated compensation range for this job at the time of posting. The actual pay rate will be based on a number of factors including skills competencies experience location and/or being pursued and other jobrelated factors permitted by law. In addition this role will be eligible for overtime pay in accordance with federal and state requirements
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No C2C or ThirdParty Vendors
Required Experience:
Senior IC
Employment Type
Unclear
