Scientist I Gene Therapy Analytical Development
Scientist I Gene Therapy Analytical Development
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Job Description
Why Sarepta Why Now
The promise of genetic medicine has arrived and Sarepta is at the forefront. We hold leadership positions in Duchenne muscular dystrophy (DMD) and limbgirdle muscular dystrophies (LGMDs) and we currently have more than 40 programs in various stages of development in gene therapy RNA and gene editing. In 2023 we launched our fourth therapy and the first ever gene therapy to treat Duchenne.
Were looking for people who see unlimited potential in themselves and who are motivated by an unwavering commitment to patients.
What Sarepta Offers
At Sarepta we care deeply about all the people in our community and believe in the importance of supporting them in all aspects of their lives. We aspire to maintain a culture that acknowledges people bring their whole selves to work and we will strive to help everyone in our community integrate their work and personal lives while maintaining productivity. We are committed to offering a range of benefits and worklife resources designed to support people in the following areas:
Physical and Emotional Wellness
Financial Wellness
Support for Caregivers
For a full list of our comprehensive benefits see our website: Importance of the Role
This individual will join a team of scientists to support the predevelopment of cell based AAV gene therapy potency assay methods at the Sarepta Andover facility. The position requires a strong background in cell culture and experience in cellbased assay method development. The individual needs demonstrable experience in managing cell lines both wild type and KO cell lines. The candidate should have hands on experience of SDSPAGE qPCR ddPCR protein extraction methods antibody screening and protein quantification methods using recombinant protein. The individual will be responsible to support analytical method development. This person will interface with the internal and external crossfunctional teams.The Opportunity to Make a Difference
- Hands on experience with cell culture to create and maintain unique mammalian wild type and KO cell lines for cellbased assays. Testing new cell lines for use in potency assays for multiple new gene therapy programs.
- Managing the production screening and testing multiple recombinant protein standards and antibodies for multiple target proteins under several gene therapy programs
- Conduct laboratory experiments involving in vitro potency methods to select the suitable cell lines antibody and recombinant proteins.
- Redesign multiple cell lines to enhance the expression of surface receptors in a reproducible manner to increase infectability
- Create and maintain clear and concise lab records and documentation.
- Author test methods predevelopment reports and study protocols.
- Support the existing development team transfer and qualification/validation activities
- Act as company liaison with CMOs to coordinate in method development and method transfer across various CMO/CRO sites as needed
More about You
- Ph.D in molecular biology cell biology or virology with at least 1 years of industrial experience.
- Working experience in a cGMP environment is preferred
- A strong understanding of AAV related cell culture methods capillary based electrophoresis molecular cloning AAV based characterization method is necessary.
- Experience in general laboratory experimentation documentation with adherence to cGMP is necessary.
- Experience in developing optimizing and transferring molecular biology test methods is useful.
- Must have effective written and verbal communication skills
- Operate as part of a team; assisting fellow lab associates in providing a functional safe working environment while maximizing laboratory efficiency
What Now
Were always looking for solutionoriented critical thinkers.
So if youre comfortable with ambiguity and candor relish challenging yourself and place kindness and integrity at the forefront of how you approach your peers and work then we encourage you to apply.
Employment Type
Full-Time
