PhD Student prestart Research Assistant mfd in Characterization of R-loop associated DNA modifications
PhD Student prestart Research Assistant mfd in Characterization of R-loop associated DNA modifications
Posted on :
11-04-2025
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Job Description
About us
The position
Get research experience with the possibility to prestart a PhD Student Position
Rloops are threestranded nucleic acid structures comprising an RNA hybrid and a displaced singlestranded DNA (ssDNA). The exposed ssDNA makes Rloops a hotspot for DNA modifications that can impact Rloop dynamics gene regulation and genomic stability (Pubmed:. Recent studies have revealed that different nucleobases exhibit specific modifications in the context of Rloops such as cytosine deamination to uracil cytosine oxidation to 5formylcytosine or guanosine oxidation to 8oxoguanine. These modifications regulate Rloop stability and hence potentially affect genome stability and gene expression. Rloopenriched modifications highlight the importance of understanding their regulatory roles and potential as therapeutic targets in diseases linked to genomic instability. The questions underlying this project are:
- How do specific DNA modifications within Rloops affect their structural stability and dynamics
- What is the relationship between Rloopassociated DNA modifications and gene regulation
- How do these modifications contribute to genomic instability and potentially to disease pathogenesis
You will employ quantitative DNARNA immunoprecipitation sequencing to map the locations and frequencies of specific DNA modifications within Rloops across various genomic contexts. You may test the impact of these modifications on Rloop stability using CRISPRCas9 to engineer cell lines with and without specific modifications. You will then observe changes in gene expression Rloop formation/disruption and cellular phenotypes. You will conduct assays to measure rates of DNA damage repair efficacy and cell viability under conditions promoting Rloop formation and modification. This will help correlate specific modifications with increased genomic instability.
See also your time as Research Assistant you will have the opportunity to apply for and transfer to the International PhD Programme (IPP).Why join us
We offer a stimulating diverse and international research environment with a pleasant working atmosphere and informal culture. The institute is modern wellequipped and centrally located with good public transport links and parking.
Our offer
- Competitive salary and favourable pension scheme
- Advanced training opportunities
- Flexible working hours
- Employee events
- Job Ticket
What else you need to know
- Starting Date:By September 2025
- Duration:initially 6 months with possibility of up to 5y PhD student position pending on successful IPP application
- Deadline:15th April 2025
- International applicants: we will assist you through the visa process and also with your application for a residence/work permit after your arrival in Mainz.
Have we sparked your interest
To apply please upload your application containing your cover letter stating research and career interests CV scans of your degrees (including copies of Abitur and Master degree if existing) to our application form on imb/jobs/scientificpositions. Should you have any questions regarding the application process please contact us by email quoting Ref. No. #CNRA02. Informal enquires should be addressed to Prof Christof Niehrs. IMB is an equal opportunity employer.
Required Experience:
IC
Employment Type
Part-Time
Key Skills
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