Research Associate
Research Associate
Posted on :
11-04-2025
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1 Vacancy
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Monthly Salary
Not Disclosed
Salary Not Disclosed
Vacancy
1 Vacancy
Posted on :
11-04-2025
Job Description
Several exciting opportunities in an earlystage life sciences company focused on developing T cell receptorbased immunotherapies.
We are seeking a highly motivated candidate to join BlueSphere Bios Tcell Receptor (TCR) Discovery team. The incumbent will use stateoftheart instrumentation and techniques for the identification of TCRs that recognize minor histocompatibility antigens as well as tumorassociated antigens and neoantigens. Additionally she/he will work with a group of individuals developing a highthroughput platform that is able to isolate and identify rare antigenspecific Tcells from healthy and patient donor samples. These positions require excellent communication skills and the ability to work effectively in a goalfocused team environment. Job level and responsibilities will be based on qualifications and experience. Candidates interested in expanding their knowledge and gaining experience in cellbased (TCRT) cancer therapies and drug discovery are highly encouraged to apply.
Qualifications
Research Associate:
- Bachelors (BS) degree in Immunology Oncology Cell Biology Molecular Biology Biological Sciences or equivalent experience required with >2 years of working experience required.
- Familiarity with 10x genomics scRNA library preparation workflow is preferred.
We are seeking a candidate for the following functional roles:
NGS Library Production
- Develop and optimize NGS library preparation workflow by utilizing nucleic acid technologies (PCR fragmentation nucleic acid cleanup)
- Establish protocols and SOP for the above techniques
- Develop and optimize LVV construction workflow utilizing molecular biology techniques (cloning plasmid purification)
- Responsible for generation of hightiter recombinant lentiviruses quality control of the lentiviral preparations and generation of associated reagents.
- Establish protocols and SOP for above molecular biology techniques
- T cell immunology includes isolation modification expansion and analysis of primary T cell populations.
- Establish protocols and SOP for T cell characterization and various assays of Tcell function.
- Maintain accurate sample and laboratory records. Document activities in electronic notebook system.
- Support TCR discovery platform operations by assisting in developing and implementing novel strategies to identify antigenspecific TCRs
- Conducting PCR cloning and colony screening assays.
- Conducting bacterial transformation propagation and purification of plasmid DNA
- Conducting lentivirus production purification and banking
- Development and implementation of coculture assays to identify novel TCRs
- Sterile culture techniques and cell sorting (including at the single cell level) to maintain and establish new cell lines for the functional characterization of TCRs
- Characterize cells using multiparameter flow cytometry and subsequent data analysis.
- Organize and conduct assigned studies independently. Notably the incumbent must be capable of troubleshooting various aspects of assay implementation.
- Work closely within the development team to advance technology platform and TCR candidates
- Assist in developing data packages and reports for internal corporate activities and regulatory submissions.
- Present regularly at team meetings.
- Other duties as assigned.
Relevant Experience and Skills
- Mammalian Cell Culture: immortalized and primary adherent and nonadherent cells
- T cell biology: Isolation and in vitro maintenance of PBMC including CD4 and CD8 primary T cells. T cell activation gene modification and expansion for downstream functional assays including TCRdextramer binding activation assays (proliferation cell based killing assays analysis of cytokine secretion etc.
- Flow Cytometry: Strong background in multicolor flow cytometry 4 channel flow cytometry analysis preferred of primary human immune cells and cell lines
- Molecular Cloning: vector design restriction enzyme analysis of expression vectors restriction independent cloning InFusion and/or Gibson
- Lentiviral technology: Understanding of LV packaging systems and LV expression vector components; experience in LV vector isolation and generation of recombinant lentivirus; lentiviral applications: transduction of immortalized and primary cells focusing on primary human PBMC and T cells
- DNA/RNA isolation and nucleic acid technologies: PCR RTPCR and QRTPCR; familiarity with NGS and RNASeq genomic DNA PCR and Sanger sequencing; familiarity with 10x genomics scRNA seq workflow; isolation of vector DNA (mini midi maxi and mega preps) agarose gel electrophoresis and DNA/RNA fragment isolation from agarose gel
- Bacterial Culture: maintenance of bacterial cells transformation of the competent bacteria isolation of vector DNA (mini midi maxi and mega preps)
- Software: flow cytometry software data analysis software (GraphPad Prism Excel etc. Microsoft Office suite
- Organizational skills: experience in working in a multidisciplinary team environment; resource management; ability to manage ones time within individual departmental and corporate goals and timelines.
Physical and Mental Requirements
- You will be expected to frequently 21 to 50 of the time):
- Lift objects from one level to another 30 lbs. or less)
- Carry objects 30 lbs. or less)
- Walk around the facility on foot
- Stand on your feet
- Ambulatory travel up to 25
- You will be expected to constantly (at least 51 of the time):
- Sit in a normal seated position
Required Experience:
IC
Employment Type
Full-Time
Key Skills
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