Technical Research Assistant I 40 Hours Day Bwh - Path-Fletcher Lab

Brigham and Womens Hospital is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race sex color religion national origin sexual orientation protected veteran status or on the basis of disability

GENERAL SUMMARY/OVERVIEW STATEMENT:

Under general supervision of Principal Investigator and in accordance with established hospital policies and procedures conducts experiments to identify new therapeutic strategies in pediatric and adult cancers (previous studies by the lab have led to FDA approval of three new for cancer). This research assistant position is cell culture intensive and uses viability protein (immunoblotting) and flow cytometry analytic techniques to evaluate tumor cell cultures after treatment with novel therapies. The position also includes responsibility (along with other lab members) for genome sequencing and other highthroughput analytics to identify therapeuticallyrelevant biologic vulnerabilities in cancer. These studies are coupled with functional validations (gene knockdowns and overexpression) to confirm and understand the therapeutic opportunities. Ideally the research assistant will look forward to preparing manuscripts for publication in highimpact science and medicine journals so strong writing ability is a plus. The research assistant will also assist in the training of new students and postdoctoral fellows in standard methods of molecular biology and cell culture. Perfect opportunity for someone planning to attend medical or graduate school in 23 years!

This laboratory is primarily interested in the biology and therapeutics of cancer.

PRINCIPAL DUTIES AND RESPONSIBILITIES:

1. Contributes to genomic and proteomic screens to identify novel therapeutic targets in human cancer. Performs tumor cell cultures and evaluates effects of treating tumor cultures with novel therapies. Carries out standard methods of molecular biology including: Isolation of various cell fractions from blood bone marrow and solid tumor specimens; immunoprecipitation and western blotting Polymerase chain reaction DNA and cDNA amplification; DNA cloning in phage and plasmids; nextgeneration sequencing; fluorescence in situ hybridization;

2. Records data summarizes and analyzes results using basic statistics.

3. Reports on progress of work at weekly laboratory seminars.

4. Prepares laboratory reagents chemicals instruments and equipment.

5. Maintains supplies inventory in accordance with department procedures.

6. Functions as a key team member in drafting and otherwise preparing highimpact publications.

7. Helps train new students and postdoctoral fellows in standard methods of molecular biology.

8. Maintains established departmental policies procedures and objectives including the areas of quality assurance safety environmental affairs and infection control.

9. Performs all other duties and responsibilities as directed.

QUALIFICATIONS:

Bachelor of Science or Bachelor of Arts degree in a biological/physical science required.

Some laboratory experience desired. Undergraduate coursework or projects may be considered as appropriate experience and may be credited upon hire if project(s) directly related to position. Undergraduate experience with Creative Writing (or other courses that focus on writing skills) is desired.

SKILLS/ABILITIES/COMPETENCIES REQUIRED:

Ability to carry out procedures in a coordinated multistep manner with appropriate consultation.

Ability to methodically record data and to analyze data using basic statistics.

Ability to contribute to manuscript drafts: specifically ability to communicate complex science concepts in written form using correct grammar and syntax.

Ability to work under general supervision.

Ability to perform a variety of routine procedures.

Ability to prioritize work.

WORKING CONDITIONS:

Normal laboratory environment. Lab contains approximately 8 workers including postdoctoral fellows residents technicians and lab aides.

Exposure to radioactive materials and human blood and tissues; occasional use of suspected carcinogens. Minimal risk when established protocols are followed.

SUPERVISORY RESPONSIBILITY:

None. Helps train new students and postdoctoral fellows in standard methods of molecular biology.